Structure of the project

Schedule:

Task 1. Selection of Biomaterials (1-9 months)

The aim is to select optimal biomaterials for biphasic and zonal osteochondral constructs for tissue engineering.

Task 2. Selection of cells (1-12 months)

The aim is to select biological factors, cell types for bony and cartilage part. The cells will be expanded in vitro, then placed appropriate scaffolds for differentiation into cartilage and bone.

Task 3. 3D Plotting of scaffold (3-21 months)

The aim is to develop a technology to fabricate bioactive 3D scaffolds with controlled external and internal geometry, chemical composition, mechanical properties and biological activity.

Task 4. Nanofibers development (3-21 months)

The aim is to develop a technology to fabricate bioactive nanofibers with required geometry and properties, characterization of the development nanofibers and biological evaluations of this nanofibers.

Task 5. 3D printing of cells (3-21 months)

The aim is to develop a technology for 3D printing of multiple cell types encapsulated in hydrogel with or without nanofibers. Moreover characterisation and biological evaluations of the developed 3D gel with cells.

Task 6. 3D osteochondral TE construct development (15-36 months)

The aim is to develop 3D biphasic and zonal TE engineered construct which could be used as in vitro models of the osteochondral defect or as a prototype of the TE product for osteochondral defect regeneration. Developed biphasic and zonal osteochondral constructs will be characterized.

Task 7. In vitro evaluation of TE constructs (21-36 months)

The aim is to develop methods for in vitro evaluation and biological evaluations of the biphasic and zonal models of osteochondral defects. The architecture of the ECM will be evaluated using SEM, TEM, micro-CT, non-linear microscopy and focused ion beam/SEM technology.

Task 8. In vivo evaluation of TE constructs (21-36 months)

The aim is to develop of the methods to in vivo evaluate of the biphasic and zonal osteochondral construct. It will be performed in a rabbit model of osteochondral lesions. Moreover biological evaluations of the biphasic and zonal osteochondral constructs will be examined. The methods to be employed are macroscopic evaluation, microscopy after decalcification and possibly electron microscopy of selected tissue samples.

questra Пестюк Чеслав Фанис Джураев Константин Мамчур Станислав Кравцов AGAM Atlantic Global Александр Прочухан Абакумов Андрей
questra Пестюк Чеслав Фанис Джураев Константин Мамчур Станислав Кравцов AGAM Atlantic Global Александр Прочухан Абакумов Андрей